Purification and properties of vaccinia virus DNA-dependent RNA polymerase.
نویسندگان
چکیده
منابع مشابه
Isolation and properties of the vaccinia virus DNA-dependent RNA polymerase.
A vaccinia virus-specified DNA-dependent RNA polymerase was isolated from the cytoplasm of infected HeLa cells. The enzyme appeared in cells only after infection; it differed in its chromatographic properties from all cellular RNA polymerases; and its subunit composition (seven subunits ranging in size from 13,500 to 135,000 daltons) was completely different from that of HeLa cell RNA polymeras...
متن کاملPurification and characterization of a DNA-dependent RNA polymerase from vaccinia virions.
A DNA-dependent RNA polymerase has been extracted from vaccinia virions and purified to near homogeneity as judged by glycerol gradient sedimentation and polyacrylamide gel electrophoresis. The native enzyme has a molecular weight of approximately 500,000 and can be dissociated into putative subunits of 140,000, 137,000, 37,000, 35,000, 31,000, 22,000, and 17,000 daltons. Activity was dependent...
متن کاملExpression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.
Encephalomyocarditis (EMC) virus RNA-dependent RNA polymerase was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST), which allowed easy purification of the fusion protein by affinity chromatography on immobilized glutathione. Inclusion of a thrombin cleavage site between the GST carrier and the viral enzyme facilitated the release of purified mature EMC viru...
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An RNA-dependent DNA polymerase was isolated from purified virions of endogenous oncornaviruses released by the MOPC-315 murine myeloma cell line. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified enzyme was found to consist of two major polypeptides with molecular weights of about 28,000 and 26,500. The active enzyme had a molecular weight of approximately 56,000, as...
متن کاملPurification and properties of DNA-dependent RNA polymerase from Bacillus subtilis vegetative cells.
A purification procedure to prepare highly purified DNA-dependent RNA polymerase from Bacillus subtilis vegetative cells is described. The enzyme consists of four different subunits, /?’, /?, (T and 01 (molecular weights 154000 for B’ and /?, 56000 for (T and 43000 for 01) in a molar ratio 1 : 1 : I : 2. By phosphocellulose chromatography RNA polymerase has been disscociated in (T subunit and c...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70799-6